Volume 2, Issue 5, September 2013, Page: 217-224
Effect of Vegetable and Animal Protein Sources on Renal Failure in Diabetic Rats
Nadia Saleh Al-Amoudi, Nutrition and Food Department, King Abdul-Aziz University, Jeddah, Saudi Arabia
Received: Aug. 2, 2013;       Published: Aug. 30, 2013
DOI: 10.11648/j.ijnfs.20130205.11      View  2839      Downloads  213
Objective: This study was carried out to determine the effects of vegetable and animal proteins on renal failure in diabetic rats. Design: We used male, adult Wistar rats [n = 90 rats] in which diabetic renal disease was induced. Intervention: Rats were fed either a control diet or diets in which the protein came from vegetable [soy, lentil, or white bean] or animal [fish, beef, chicken, or eggs] sources. Main outcome measures: Urinary levels of total lipids, triglycerides, cholesterol fractions, liver enzymes function, total glucose, bilirubin, sodium [Na], potassium [K], albumin, creatinine and urea nitrogen were determined. Results: The fish protein and white bean protein diets exhibited the best results for lipid profiles in total lipid and triglycerides on renal failure in diabetic rats. Total cholesterol was decreased in the fish and soy protein diets. The serum liver enzyme activities of aspartate and alanine amino transferases showed that all protein sources appeared to perform better than the control group with respect to enzyme activities. However, the fish protein group exhibited the best results. In terms of the effects of the different protein sources on glucose [Glu], the fish protein group exhibited the lowest mean Glu level of all of the groups studied. Moreover, these levels were not significantly different from those of the white bean protein group compared with both control groups. The animal protein groups excreted higher urine volumes and more albumin than the plant protein groups. The creatinine and urea nitrogen levels in the fish protein group were similar to those of the soy group. The white bean protein group exhibited the highest Na levels among the experimental groups, whereas the egg protein group exhibited the lowest Na levels. Conclusion: A diet containing low protein fish may slow the progression of chronic renal failure in diabetic rats.
Rat, Dietary Protein, Diabetes, Nephropathy
To cite this article
Nadia Saleh Al-Amoudi, Effect of Vegetable and Animal Protein Sources on Renal Failure in Diabetic Rats, International Journal of Nutrition and Food Sciences. Vol. 2, No. 5, 2013, pp. 217-224. doi: 10.11648/j.ijnfs.20130205.11
Gross JL, de Azevedo MJ, Silveiro SP, Canani LH, Caramori ML, Zelmanovitz T. Diabetic Nephropathy: Diagnosis, Prevention, and Treatment. Diabetes Care. 2005; 28:164-176.
Parving HH, Smidt UM, Friisberg B, Bonnevie-Nielsen V, Andersen AR. A prospective study of glomerular filtration rate and arterial blood pressure in insulin-dependent diabetics with diabetic nephropathy. Diabetologia. 1981; 20:457-461.
Viberti GC, Bilous RW, Mackintosh D, Keen H. Monitoring Glomerular Function in Diabetic Nephropathy. The Am J Med. 1983;74:256-264.
Finne P, Reunanen A, Stenman S, Groop PH, Gronhagen-Riska C. Incidence of end-stage renal disease in patients with type 1 diabetes. JAMA 2005; 295:1782-1787.
Bilous RW. The kidney in systemic disease. In: Warrell DA, Cox TM, Firth JD, Benz EJ, eds. Oxford Textbook of Medicine. Oxford: Oxford University Press; 2005.
Connor H, Annan F, Bunn E, Forst G, McGough N, Sarwar T, Thomas B. The implementation of nutrition advice for people with diabetes. Diabet. Med. 2003; 20:789-807.
Toeller M, Klischan A, Heitkamp G, Schumacher W, Milne R, Buyken A, Karamanos B, Gries FA. Nutritional intake of 2868 IDDM patients from 30 centers in Europe. Diabetologia. 1996; 39:929-939.
Ikizler TA, Greene JH, Wingard RL. [1995].Spontaneous dietary protein intake during progression of chronic renal failure. Am J Nephrol. 1995;6:1386-1391.
El Nahas AM, Masters-Thomas A, Brady SA. Selective effect of low protein diets in chronic renal diseases. Brit Med J. 1984;289:1337-1341.
Rosman JB, Meijer S, Sluiter WJ, Wee PMT, Piers-Becht TPM, Donker AJM. Prospective randomised trial of early protein restriction in chronic renal failure. Lancet; December. 1984;1291-1296.
Klahr S, Levey AS, Beck GJ. The effects of dietary protein restriction and blood pressure control on the progression of chronic renal diseases. New Eng J Med. 1994;330:884.
Levey AS, Beck GJ, Caggiula AW. Trends towards a beneficial effect of a low protein diet during additional follow-up in the Modification of Diet in Renal Disease Study. Am J Nephrol. 1994; 5:336A.
AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists, 18th ed., Washington, DC, 2005.
Farombi OE, Ekott M. Cureuminatteruates gentamicin induced renal oxidative damage in rats. Food and Chemical Toxicology, 2006; 44:1443-1448.
Madar Z. Effect of brown rice and soybean dietary fiber on the control, glucose, and lipid metabolism in diabetic rats. Am Med J Clin Nutr. 1983; 388-393.
Tietz NW. Text Book of Clinical Chemistry. P.796.Saunders, WB. Co., London-Philadelphia; 1986.
Knight JA, Anderson S, and James MR. Chemical basis of the sulfophospho vanillin reaction for estimating total serum lipids. Clin Chem. 1972; 18[3]: 199-202.
Allain CC, Poor LS, Chan SO. Enzymatic determination of total serum cholesterol. Clin Chem. 1974; 20[4]:470-475.
Fossati P, Prencipe L. The determination of triglyceride using enzymatic methods. Clin Chem. 1982; 28:2077-2081.
Burstein M. HDL Cholesterol determination after separation of high density lipoprotein. Lipids Res. 1970; 11:583-589.
Fruchart JC. LDL Cholesterol determination after separation of low density lipoprotein. Rev Fr Des Laboratory. 1982; 103:7-17.
Lee R, Nieman D. Nutritional Assessment. 2nd Edition. Mosby, Missouri, USA; 1996.
Bergmeyer HU, Horder M, Rej R. Approved recommendation on IFCC method for the measurement of catalytic concentration of enzyme. Part 2. J Clin Chem. 1985; 24[7]:497-510.
Doumas BT, Watson WA, Biggs HG. Albumin standard and measurement of serum albumin with bromocresol green. Clin Chem. 1971; 31:87-92.
Schirmeister J. Creatinine standard and measurement of serum creatinine with picric acid Dtsch Med Wschr. 1964; 89:1018-1022.
Patton CH, Crouch SR. Enzymatic colorimetric method to determine urea in serum. Anal Chem. 1977; 49:464-469.
Henry RJ. Clinical Chemist. Principles and Techniques, 2nd Edition, Hagerstown [MD], Hareer, Row. 1974; PP: 882.
Vinchi F, Gastaldi S, Silengo L, Altruda F, Tolosano E. Hemopexin prevents endothelial damage and liver congestion in a mouse model of heme overload. Am J Pathol. 2008;173[1]: 289–299.
Armitage P, Berry G. Statistical Method in Medical Research. Blackwell, Oxford, UK, 1987; PP: 93-213.
Waller WM, Duncan DB. A bays role for symmetric multiple composition problem. Am Sate Assoc J. 1969; 65:1485-1492.
Giboney MJ, Kritchevsky D. Animal, and Vegetable Proteins in Lipid Metabolism and Atherosclerosis. Current Topics in Nut & Dis Vol 8, PP: 177. New York, Alan R. Liss Inc; 1983.
Lovati MR, West C, Sirtori C, Beynen A. [1990]. Dietary animal proteins and cholesterol metabolism in rabbits Brit J Nutr. 1990; 64:473-485.
Carroll KK.[1978]. The role of dietary protein in hypercholesterolemia and atherosclerosis. Lipids. 1978; 13:360-365.
Kyuyvenhoven MW, West CE, Vander Meer R, Beynen AC. Fecal steroid excretion in relation to the development of casein-induced hypercholesterolemia in rabbit. J Nutr. 1986; 116:1395-1404.
Sirtori CR, Agradi F, Mantero O, Conti F, Gatti E. Soy bean protein diet in the treatment of type II hyperlipoproteinaémia. Lancet. 1977; I: 275-277.
Anderson JW, Johnstone BM, Cook-Newell ME. Meta-analysis of the effects of soy protein intake on serum lipids. N Engl J Med. 1995; 333:276-282.
Jenkins DJ, Kendall CW, Vidgen E. High-protein diets in hyperlipidemia: Effect of wheat gluten on serum lipids, uric acid, and renal function. Am J Clin Nutr. 2001; 74:57-63.
Sacks F, Lichtenstein A, Van Horm H, Harris W, Kris-Etherton P, Winston M. Soy protein, isoflavones and cardiovascular health: An American Heart Association Science Advisory for Professionals from the Nutrition Committee. Circulation, 2006; 113:1034-1044.
Margetts BM, Beilin LJ, Vandongen R, Armstrong BK. Vegetarian diet in mild hypertension: A randomized controlled trial. Br J Med. 1986; 293:1468-1471.
Wiseman MJ, Hunt R, Goodwin A, Gross JL, Keen H, Viberti DC. Dietary composition and renal function in healthy subject. Nephron. 1987; 46:37-42.
Massey L. Vegetarian diet may not be better- Renal and Urology News available at 2007.
Teixeria SR, Tappenden KA, Carson L, Jpnes R, Parbhudesai M, William P, Erdman JW. Isolated soy protein consumption reduces urinary albumin excretion and improves the serum lipid profile in men with type 2 diabetes mellitus and nephropathy. J Nutr. 2004; 134:1874-1880.
Cianciaruso B, Capuano AD, Amaro E, Ferrara N, Nastasi A, Conte G, Bellizzi V, Andreucci VE. Dietary compliance to a low protein and phosphate diet in patients with chronic renal failure. Kidney International. 1989; 36:173-176.
Jibani MM, Bloodworth LL, Foden E, Griffiths KD, Galpin OP. Predominantly vegetarian diet in patinets with incipient and early clinical diabetic nephropathy: effects on albumin excretion rate and nutritional status. Diab Med. 1991; 8:949-953.
Pecis M, de Azevedo MJ, Gross JL. Chicken and fish diet reduced glomerular hyperfiltration in IDDM patients. Diabetes Care. 1994; 17:665-72.
Meloni C, Morosetti M, Suraci C, Pennafina MG, Tozzo C, Taccone-Gallucci M, Casciani CU.[2002]. Severe dietary protein restriction in overt diabetic nephropathy: benefits or risks?. J Ren Nutr. 2002; 12:96-101.
Meloni C, Tatangelo P, Cipriani S, Rossi V, Suraci C, Tozzo C, Rossini B, Cecilia A, Di Franco D, Straccialano E, Casciani CU. Adequate protein dietary restriction in diabetic and nondiabetic patients with chronic renal failure. J Ren Nutr. 2004; 14:208-213.
Raine AEG. The rising tide of diabetic nephropathy - the warning before the flood? Nephrology Dialysis and Transplantation. 1995; 10:460-461.
Robertson LM, Waugh N, Robertson A. Protein restriction for diabetic renal disease. Cochrane Database of Systematic Reviews, Issue 4. Art. No.: CD002181. DOI: 10.1002/14651858.CD002181.pub2. 2007.
Gin H, Rigalleau V, Aparicio M. Which diet for diabetic patients with chronic renal failure?. Nephrol Dial Transplant. 1999; 14[11]: 2577-2579 .
Nedredal GI, Amiot BP, Nyberg P, Luebke-Wheeler J, Lillequard JB, Mckenzie JJ, Nyberg SL. Optimization of mass transfer for toxin removal and immunoprotection of hepatocytes in a bioartificial liver. Biotechnol Bioeng. 2009; 104[5]:995-1003.
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