Volume 4, Issue 1, January 2015, Page: 45-50
Optimization of GSH Extraction Technology from Germinating Soybean Using Response Surface Methodology
Guifeng Li, Department of Food Science and Engineering of Shanxi Normal University, Linfen, China
Limin Dong, Department of Food Science and Engineering of Shanxi Normal University, Linfen, China
Junli Xu, Department of Food Science and Engineering of Shanxi Normal University, Linfen, China
Received: Jan. 5, 2015;       Accepted: Jan. 20, 2015;       Published: Feb. 2, 2015
DOI: 10.11648/j.ijnfs.20150401.17      View  2370      Downloads  134
Abstract
In order to establish optimal water extraction technology of GSH from soybean, response surface method, based on Box–Behnken design, was used to optimize extraction conditions. Crude GSH including in protein was separated by Sephadex G-50 chromatography method. A second order quadratic mathematical model for GSH in soybean extraction was established. Its validity was verified, and the alternate action of the above three factors and their optimum level ranges were investigated. Results showed that the optimum conditions to achieve the maximum extraction rate were as follows: pH of extraction liquid 7.62, extraction temperature 71.91℃, and extraction time 135.67min. Under such conditions, extraction rate of GSH reaches 1.063% (the relative error 1.1%), which was well matched with the predicted yield.
Keywords
Soybean, GSH, Extraction Technology, Molecular Weight, RSM
To cite this article
Guifeng Li, Limin Dong, Junli Xu, Optimization of GSH Extraction Technology from Germinating Soybean Using Response Surface Methodology, International Journal of Nutrition and Food Sciences. Vol. 4, No. 1, 2015, pp. 45-50. doi: 10.11648/j.ijnfs.20150401.17
Reference
[1]
Sarvajeet SG., Naser AA., Mirza H., Ritu G., Dipesh KT., Iqbal A., Eduarda P., Narendra T.. Glutathione and glutathione reductase: A boon in disguise for plant abiotic stress defense operations. Plant Physiology and Biochemistry, 70, 2013, pp. 204-212. DOI 10. 1016/j. ppb. 2013. 05. 032.
[2]
Noctor, AG., Mhamdi S., Chaouch Y., Han Y., Neukermans J., Queval G.. Glutathione in plants: an integrated overview, Plant Cell Environ. 35, 2012, pp. 454-484. DOI 10. 1111/j. pce. 2011. 02. 400.
[3]
Danyelle M., Townsend, Kenneth DT., Haim T.. The importance of glutathione in human disease [J]. Biomedicine & Pharmacotherapy, 57, 2003, pp. 145-155. DOI 10. 1016/j. bp. 2003. 03. 043.
[4]
Queval, A. Mhamdi, S. Chaouch, C H (2011) Glutathione, vol. 9, The Arabidopsis Book/American Society of Plant Biologists.
[5]
Hossein S., Mohammad MN., Hossein VD., Shakeri M., Towhidi A., Shahneh AZ., Zhandi, M.. Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing [J]. Small ruminant research, 112, 2012, pp. 123-127. DOI 10. 1016/j. srr. 2012. 12. 015.
[6]
Xu YY., Cai SS., Wu HC., Yu j.. Optimization on ultrasonic extraction of phytosterols from soybean by response surface methodology. Food Research and Development, 35, 2014, pp. 36-39. DOI 10. 3969/j. frd. 2014. 09. 09.
[7]
Xu LP., Yang CH., Wang X.. Condition optimization of extracting glutathione from Corn germ by response surface methodology. Journal of the Chinese Cereals and Oils Association, 25, 2010, pp. 15-18. DOI 10.1074/j. jccoa. 2010. 05. 0015.
[8]
Chen L, Yuan H L, Chen D L. Comparative study on methods for determination of glutathione in fermentation liquor. Physical Testing and Chemical Analysis (Part B:Chemical Analysis), 51, 2010, pp. 876-878. DOI 10. 4020/j. ptca. 2010. 08. 0876.
[9]
Wei GY., Li Y., Du GG., Chen J.. Effects of dissolved oxygen and pH on candida utilis batch fermentation of glutathione. Chinese Journal of Biotechnology, 19, 2003, pp. 734-739.DOI 10. 3061/j. cjb. 2003. 06. 0734.
[10]
Li GF., Zhao GJ., Xiao CL., Liu XH.. Optimization of Enzymolysis Technology of Flammulina velutipes proteins and evaluation of Peptides antibacterial activity. Journal of Chinese Institute of Food Science and Technology, 12, 2012, pp. 108-114. DOI 10. 7848/j. jcifst. 2012. 10. 0107.
[11]
Wu XT., Zhu T., Zheng QM., Sheng C.. Study on extraction of sweet Chinese Yam skin saponins by using response surface methodology. Journal of the Chinese Cereals and Oils Association, 26, 2011, pp. 92-96. DOI 10. 0174/j. jccoa. 2011. 06. 0091.
Browse journals by subject