Volume 4, Issue 1, January 2015, Page: 69-74
Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes
Byoung-Mok Kim, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea; Division of Metabolism and Functionality Research, Korea Food Research Institute, Seongnam, Korea
Heang-Jeun Heo, Division of Metabolism and Functionality Research, Korea Food Research Institute, Seongnam, Korea
Dong-Soo Kim, Jeonbuk Institute for Bioindustry, Jeonju, Korea
Young-Myung Kim, Gi-jang Mulsan Col., Busan, Korea
In-Hack Jeong, Division of Marine Food Bioscience & Technology, Gangneung National University, Gangneung, Korea
Young-Min Chi, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea
Received: Jan. 19, 2015;       Accepted: Feb. 1, 2015;       Published: Feb. 9, 2015
DOI: 10.11648/j.ijnfs.20150401.20      View  2451      Downloads  121
Abstract
In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.
Keywords
Lipolytic Effect, Eumicrotremus Orbis, 3T3-L1 Adipocytes, Anti-Obesity, Marine Resource
To cite this article
Byoung-Mok Kim, Heang-Jeun Heo, Dong-Soo Kim, Young-Myung Kim, In-Hack Jeong, Young-Min Chi, Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes, International Journal of Nutrition and Food Sciences. Vol. 4, No. 1, 2015, pp. 69-74. doi: 10.11648/j.ijnfs.20150401.20
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