Volume 4, Issue 4, July 2015, Page: 465-476
Protective Effect of Nutmeg and Rosemary on Oxidative Stress in Hypercholesterolemic Rats
Naglaa H. M. Hassanen, Special Food and Nutrition Department, Food Technology Research Institute, Agriculture Research Center, Giza, Egypt
Received: Apr. 25, 2015;       Accepted: May 13, 2015;       Published: Jul. 1, 2015
DOI: 10.11648/j.ijnfs.20150404.18      View  4710      Downloads  129
The present work was conducted to study the effect of nutmeg (Myristica fragrans) and rosemary (Rosmarinus officinalis) spices or their essential oils on the nutritional parameters of rats suffering from hypercholesterolemic. Fifty-four male rats weighting approximately 300 grams were divided into nine groups, each group containing six rats. Group H1 fed on basal diet as a control negative group. Group H2 fed on diets containing 15% of beef tallow instead of the fresh sunflower oil proportion and allowed added 1 % of H2O2 in the drinking water to increase the oxidative stress and the other groups H4, H5, H6 , H7, H8 and H9 fed on the same diet used in group H2 and supplemented with different treatments nutmeg , rosemary and mixture powders groups H4, H5, H6 (2g / 100 g diet) , respectively. Meanwhile, groups H7, H8 and H9 fed on the same diet used in group H2 and supplemented with different treatments nutmeg , rosemary and mixture essential oils (0.6gm / 100 gm fat) , respectively. Group H3 fed on the same diet used in group H2 and supplemented with (drug) Lipitor Atorvastatin(20 mg/Kg BW rat/day by stomach tube). After 8 weeks the rats feeding with treatments and hypercholesterolemic diet rats significantly decreased levels of glucose. The treatment also resulted in a significant improvement in lipid profile, oxysterol levels, liver function, and kidney function. However, a significantly increment in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reduced (GSH) were observed in blood of hypercholesterolemic rats treated with nutmeg, rosemary powders or their essential oils. The treated groups showed a significant decrement in thiobarbituric acid reactive substances (MDA) in serum. Since the study of induction of the redox enzymes is considered to be a reliable marker for evaluating the antiperoxidative efficacy of the spices. Treatment with nutmeg, rosemary powders or their essential oils reduces the histopathological, heart, liver and kidney abnormalities associated with hypercholesterolemic.
Nutmeg, Rosemary, Essential Oils, Oxysterol, Liver Function, Kidney Function, MDA, Histopathological
To cite this article
Naglaa H. M. Hassanen, Protective Effect of Nutmeg and Rosemary on Oxidative Stress in Hypercholesterolemic Rats, International Journal of Nutrition and Food Sciences. Vol. 4, No. 4, 2015, pp. 465-476. doi: 10.11648/j.ijnfs.20150404.18
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