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Volume 5, Issue 2, March 2016, Page: 139-144
Watermelon Seeds as Food: Nutrient Composition, Phytochemicals and Antioxidant Activity
Betty Tabiri, Department of Food Science and Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Jacob K. Agbenorhevi, Department of Food Science and Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Faustina D. Wireko-Manu, Department of Food Science and Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Elsa I. Ompouma, Department of Food Science and Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Received: Mar. 7, 2016;       Accepted: Mar. 18, 2016;       Published: Mar. 30, 2016
DOI: 10.11648/j.ijnfs.20160502.18      View  27786      Downloads  833
Watermelon (Citrullus lanatus) seeds are often discarded while the fruit is eaten. In this study, seeds of three (3) varieties of watermelon (Charleston gray, Crimson sweet and Black diamond) were analyzed for their proximate, minerals, phytochemicals, total phenols content and antioxidant activity. The proximate analysis and phytochemicals screening were performed using standard procedures whereas minerals content was determined by atomic absorption spectrophotometry. DPPH free radical scavenging activity and Folin-ciocalteau assays were used to determine antioxidant activity and total phenol content, respectively. The results indicated that the watermelon seeds had moisture content in the range of 7.40 - 8.50%; fat, 26.50 - 27.83%; protein, 16.33 - 17.75%; fibre, 39.09 - 43.28%; ash, 2.00 - 3.00%; carbohydrate, 9.55 - 15.32% and energy value of 354.05 - 369.11 kcal/100g. The seeds also contained appreciable minerals (Ca, P, Mg, Na, K and Zn) with K (3.40-3.5 mg/100g) being the highest while Na (0.07 - 0.08 mg/100g) was the least. DPPH% inhibition varied between 59.88-94.46% inhibition with trolox equivalent of 82.59-130.29 µM/g depending on the variety. Saponins, tannins, triterpenoids glycosides and alkaloids were present in all samples. Crimson sweet seeds had the highest total phenol content (5416 mgGAE/100g), followed by Black diamond (3949 mg GAE/100g) and the least, Charleston gray (1494 mg GAE/100g). Similarly, Crimson sweet had the highest antioxidant activity, followed by Black diamond and lastly, Charleston gray. The present findings suggest watermelon seeds as considerable source of nutrients in the diet and may have health and economic benefits due to its fibre, minerals, phenolics content and antioxidant activity.
Citrullus lanatus, Proximate Composition, Minerals, Total Phenols, Free Radical Scavenging Activity
To cite this article
Betty Tabiri, Jacob K. Agbenorhevi, Faustina D. Wireko-Manu, Elsa I. Ompouma, Watermelon Seeds as Food: Nutrient Composition, Phytochemicals and Antioxidant Activity, International Journal of Nutrition and Food Sciences. Vol. 5, No. 2, 2016, pp. 139-144. doi: 10.11648/j.ijnfs.20160502.18
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ministry of Food and Agriculture (2011). Facts sheet on watermelon production. Ghana.
Okonmah, L. U., Agbogidi, O. M. and Nwagu, O. K. (2011) Evaluation of four varieties of watermelon (critrullus lanatus thumb) in Asaba agro-ecological environment. International Journal of Advanced Biological Research. 1(1), 126-130.
Oyeleke, G. O. 1, Olagunju, E.O. and Ojo, A: (2012) Functional and Physicochemical Properties of Watermelon (Citrullus Lanatus) Seed and Seed-Oil. Journal of Applied Chemistry, 2(2), 29-31.
Braide W., Odiong, I. J. and Oranusi S. (2012) Phytochemical and Antibacterial properties of the seed of watermelon (Citrullus lanatus). Prime Journal of Microbiology Research, 2(3), 99-104.
Jensen B. D., Toure, F. M., Hamattal, M. A., Toure, F. A. and Nantoumé D. A. (2011). Wtermelons in the Sand of Sahara: Cultivation and use of indigenous landraces in the Tombouctou Region of Mali, Ethnobotany Research and Applications, 9, 151-162.
Trease, G. E. and Evans, W. C. (2002). A text book of pharmacognosy, 15th edition. Academic press, London.
Tiwari, P., Kumar, B., Kaur, M., Kaur, G. and Kaur, H. 2011. Phytochemical screening and Extraction: A Review. Internationale Pharmaceuticasciencia 1 (1), 98-106.
Boakye, A. A., Wireko-Manu, F. D., Agbenorhevi, J. K. and Oduro, I. (2015) Antioxidant Activity, Total Phenols and Phytochemical Constituents of four Underutilised Tropical Fruits, International Food Research Journal, 22(1), 262-26.
Dossou, V. M. Agbenorhevi, J. K., Combey, S. and Afi-Koryoe, S., (2014) Ackee (Blighia sapida) Fruit Arils: Nutritional, Phytochemicals and Antioxidant Properties, International Journal of Nutrition and Food Sciences, 3(6), 534-537.
Agbenorhevi, J. K. and Marshall L. J. (2012) Investigation into the total phenols and antioxidant activity during storage of fruit smoothies. Journal of Food Science and Engineering, 2, 72-79.
Lakshmi, J. and Purnima K. (2011). Nutritional potential, bioaccessibility of minerals and functionality of watermelon (Citrullus vulgaris) seeds. LWT - Food Science and Technology, 44, 1821- 1826.
FAO (1982), Food composition table for the near East nuts and seeds, FAO Food and Nutrition Paper 26.
Madaan, T. R. and Lai, B. M. (1984). Some studies on the chemicalcomposition of Cucurbit kernels and their seed coats. Plant Foods for Human Nutrition, 34: 81-86.
Loukou A. L., Gnakri D., Dje Y., Kippre A. V., Malice M., Baudoin J. P., Zoro Bi I.A. (2007) Macronutrient Composition of Three Cucurbit Species Cultivated for Seed Consumption in Cote D’lvoire. African Journal of Biotechnolology, 6 (5), 529-533.
Mabalaha, M. B., Y. C. Mitei and S. O. Yoboah, (2007) A Comparative study of the properties of selected melon seeds oils as potential candidates for development into commercial edible vegetable oil. Journal of American Oil Chemists’ Society, 84: 31-34.
Hassan, L. G., Dangoggo, S. M. Umar, K. J. Saidu, I. and Folorunsho, F. A. (2008). Proximate, Minerals and Anti-nutritional Factors of Daniellia oliveri seed kernel. Chemclass Journal, 5, 31-36.
Özcan M., (2009). Some Proximate Characteristics of Fruit and Oil of Walnut (Juglansregia L.) Growing in Turkey, Iranian Journal of Chemistry & Chemical Engineering, 28(1), 57-62.
Jack T. J. (1972). Cucurbit Seeds: Characterizations and Uses of Oils and Proteins. A Review. Economic Botany 26(2): 135–141.
Wilcox, B. F. (2006). Fundamentals of Food Chemistry.1st Edition, Paraclete, Nigeria, pp 124-16.
Ojieh G. C., Oluba O. M., Ogunlowo Y. R., Adebisi K. E., Eidangbe G. O. and Orole R.T. (2008). Compositional studies of Citrulluslanatus (Egusii melon) Seed. The Internet Journal of Nutrition and Wellness. Vol. 6 No 1.
Acar, R., Özcan, M. M., Kanbur, G. and Dursun, N. (2012). Some physic-chemical properties of edible and forage watermelon seeds, Iran. J. Chem. Chem. Eng., 31(4), 41-47.
Oseni, O. A and Okoye, V. I.: (2013) Studies of Phytochemical and Antioxidant properties of the Fruit of Watermelon (Citrullus lanatus). Journal of Pharmaceutical and Biomedical sciences; 27(27): 508-514. (Article no 14).
Samia F. El-Safy, Rabab, H. Salem and Abd El-Ghany, M.E. (2012): Chemical and Nutritional Evaluation of Different Seed Flours as Novel Sources of Protein, World Journal of Dairy & Food Sciences ,7 (1),: 59-65, 2012.
Arapitsas P.: (2012) Hydrolyzable tannin analysis in food. Elsevier Food Chemistry. Food Chemistry 135 1708–1717.
Abdul-Mumeen I.: (2013). Biochemical and Microbiological analysis of shea nut cake: A waste product from shea butter processing. MPhil. Thesis, Kwame Nkrumah University of Science and Technology.
Varadharajan V., Janarthanan U. K., Vijayalakshmi K. (2012) Physicochemical, Phytochemical screening and Profiling of secondary metabolites of annona squamosa leaf extract. World Journal of pharmaceutical research. 1(4), 1143-1164.
Tiwari P., Kumar B., Kaur M, Kaur G and Kaur H. (2011) Phytochemical Screening and Extraction Review. Internationale Pharmaceutica Sciencia. 1(1), 98-106.
Oseni, O. A. and Okoye, V. I.: (2013) Studies of Phytochemical and Antioxidant properties of the Fruit of Watermelon (Citrullus lanatus). Journal of pharmaceutical and biomedical sciences; 27(27): 508-514.
Francisco, I. A. and Pinotti M. H. P.: (2000) Cynogenic glycosides in plants. Brazilian Archives of Biology and Technology, 43(5). 487-492.
Johnson J. T., Iwang E. U., Hemen J. T., Odey M. O., Efiong E.E. and Eteng, O. E.: (2013) Evaluation of anti-nutrient contents of watermelon Citrullus lanatus. Annals of Biological Research, 3 (11): 5145-5150.
Joshi, H. and Jain, P. K. (2012) Coumarin: Chemical and Pharmacological Profile. Journal of Applied Pharmaceutical Science 2(6), 236-240.
Rahman H., Manjulak K., Anoosha T., Nagaveni K., Chinna E.M. and Dipankar B.: (2013) In-vitro antioxidant activity of citrullus lanatus seed extracts. Asian Journal of Pharmaceutical and Clinical Research. 6(3), 152-157.
Duda-Chodak A. and Tarko T. (2007) Antioxidant properties of different fruit seeds and peels. Acta Scientiarum Polonorum 6(3), 29-36.
Mohammed I. S. A., Motaal A. A. and Ludger B. (2011) Total Phenolic Content and Antioxidant Activity of Standardized Extracts from Leaves and Cell Cultures of Three Callistemon Species. American Journal of Plant Sciences, 2, 847 850.
Rodriguez E. B., Flavier, M. E., Rodriguez-Amaya D. B. and Amaya-Farfan J. (2006) Phytochemicals and functional foods. Current situation and prospect for developing countries. Segurança Alimentar e Nutricional, Campinas, 13(1): 1-22.
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