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Volume 5, Issue 3, May 2016, Page: 170-174
Effect of Grape Juice Consumption and Red Wine in the Osteoprotegerin Level and Systolic Blood Pressure in Rats Wistar Fed High - Fat Diet
Teresa Palmisciano Bedê, Department of Nutrition and Dietetics, Federal Fluminense University, Niteroi, Brazil
Vânia Mattoso, Department of Nutrition and Dietetics, Federal Fluminense University, Niteroi, Brazil
Ana Cláudia Pascoal, Department of Nutrition and Dietetics, Federal Fluminense University, Niteroi, Brazil
Eduardo de Salvo Castro, Department of Nutrition and Dietetics, Federal Fluminense University, Niteroi, Brazil
Carlos Alberto Soares da Costa, Department of Nutrition and Dietetics, Federal Fluminense University, Niteroi, Brazil
Gilson Teles Boaventura, Department of Nutrition and Dietetics, Federal Fluminense University, Niteroi, Brazil
Vilma Blondet de Azeredo, Department of Nutrition and Dietetics, Federal Fluminense University, Niteroi, Brazil
Received: Mar. 21, 2016;       Accepted: Mar. 31, 2016;       Published: Apr. 16, 2016
DOI: 10.11648/j.ijnfs.20160503.13      View  4221      Downloads  190
High-fat diet contributes to reduction of bone mineral density and development of cardiovascular diseases. High-fat diet promotes the release substrate for formation of atheromatous plaques as well as increase production of cytokines and prostaglandins, interfering negatively in the concentration of osteoprotegerin - responsible for inhibiting the osteoclastogenesis. The interest by functional foods and their effects on diseases prevention is growing. This study evaluated the effect of whole grape juice consumption and red wine in the osteoprotegerin level and blood pressure in rats fed fat diet. They were used 40 rats Wistar Albino, adult, divided in groups: control - balanced diet; hiperlipydic – high-fat diet; wine - red wine + high-fat diet; juice - grape juice + high-fat diet. Water and ration were offered ad libitum and grape juice and red wine daily, during 60 days. Blood pressure was measured by a tail plestimograph and interleukin 6 (IL-6) and osteoprotegerin by ELISA. Results are expressed as mean and standard deviation. For comparison it was used ANOVA and Tukey as post-test, considering p<0.05. Systolic blood pressure (mmHg) was lower in grape juice group than hiperlipydic and red wine groups and similar to control group. IL-6 level (pg/mL) was lower in grape juice group than wine group, but it was similar to control and hiperlipydic groups. Hiperlipydic and wine groups showed lower osteoprotegerin level (pg/mL) than control and juice groups. Daily consumption of grape juice seems minimize the effects of high-fat diet in cardiovascular diseases risk and bone demineralization, by maintaining adequate concentration of osteoprotegerin and the systolic blood pressure in animals.
Polyphenols, Grape Juice, Red Wine, High - Fat Diet, Blood Pressure, Interleucin 6, Osteoprotegerin, Wistar Rats
To cite this article
Teresa Palmisciano Bedê, Vânia Mattoso, Ana Cláudia Pascoal, Eduardo de Salvo Castro, Carlos Alberto Soares da Costa, Gilson Teles Boaventura, Vilma Blondet de Azeredo, Effect of Grape Juice Consumption and Red Wine in the Osteoprotegerin Level and Systolic Blood Pressure in Rats Wistar Fed High - Fat Diet, International Journal of Nutrition and Food Sciences. Vol. 5, No. 3, 2016, pp. 170-174. doi: 10.11648/j.ijnfs.20160503.13
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Levy, R. B., Claro, R. M., Mondini, L., Sichieri, R. and Monteiro, C. A. Distribuição regional e socioeconômica da disponibilidade domiciliar de alimentos no Brasil em 2008-2009. Rev. Saúde Pública. Vol. 46, Nº 1, 2012, pp. 6-15.
Zhang, X., Dong, F., Ren, J., Driscoll, M. J. and Culver, B. High dietary fat induces NADPH oxidase-associated oxidative stress and inflammation in rat cerebral cortex. Experimental neurology. Vol. 191, Nº 2, 2005, pp. 318-325.
De Souza, C. T., Araujo, E. P., Bordin, S., Ashimine, R., Zollner, R. L., Boschero, A. C., and Velloso, L. A. Consumption of a fat-rich diet activates a proinflammatory response and induces insulin resistance in the hypothalamus. Endocrinology. Vol. 146, Nº 10, 2005, pp.4192-4199.
Morony, S., Tintut, Y., Zhang, Z., Cattley, R. C., Van, G., Dwyer, D.... and Demer, L. L. Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr (−/−) mice. Circulation. Vol. 117, Nº 3, 2008, pp. 411-420.
Parhami, F., Tintut, Y., Beamer, W. G., Gharavi, N., Goodman, W., and Demer, L. L. Atherogenic High‐Fat Diet Reduces Bone Mineralization in Mice. Journal of Bone and Mineral Research. Vol. 16, N 1, 2001, pp. 182-188.
Santana, A. B. C., Souza Oliveira, T. C. D., Bianconi, B. L., Barauna, V. G., Santos, E. W. C. O., Alves, T. P., .. and Krieger, J. E. Effect of high-fat diet upon inflammatory markers and aortic stiffening in mice. BioMed research international. Vol. 2014, 2014.
Fyhrquist, F., and Saijonmaa, O. Renin‐angiotensin system revisited. Journal of internal medicine. Vol. 264, Nº 3, 2008, pp. 224-236.
Mackie, E. J. Osteoblasts: novel roles in orchestration of skeletal architecture. The international journal of biochemistry & cell biology. Vol. 35, Nº 9, 2003, pp. 1301-1305.
Cerri, P. S. Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla. The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology. Vol. 286, Nº 1, 2005, pp. 833-840.
Yasuda, H., Shima, N., Nakagawa, N., Yamaguchi, K., Kinosaki, M., Mochizuki, S. I.... and Suda, T. Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANK. Proceedings of the National Academy of Sciences. Vol. 95, Nº 7, 1998, pp. 3597-3602.
Vogel, P., Machado, I. K., Garavaglia, J., Zani, V. T., de Souza, D., and Morelo, S. D. B. Polyphenols benefits of olive leaf (Olea europaea L) to human health. Nutrición hospitalaria: Organo oficial de la Sociedad española de nutrición parenteral y enteral. Vol. 31, Nº 3, 2015, pp. 1427-1433.
Lucile Tiemi, A. B. E., da Mota, R. V., Lajolo, F. M., and Genovese, M. I. Compostos fenólicos e capacidade antioxidante de cultivares de uvas Vitis labrusca L. e Vitis vinifera L. Ciênc. Tecnol. Aliment. Vol. 27, Nº 2, 2007, pp. 394-400.
Qi, L., Shen, H., and Ordovas, J. M. Hearts and bones. Nutrition, Metabolism and Cardiovascular Diseases. Vol. 13, Nº 3, 2003, pp. 165-174.
Ni, Z., Wang, X. Q., and Vaziri, N. D. Nitric oxide metabolism in erythropoietin-induced hypertension effect of calcium channel blockade. Hypertension. Vol. 32, Nº 4, 1998, pp. 724-729.
Duarte, A. C. G. D. O., Fonseca, D. F., Manzoni, M. S. J., Soave, C. F., Sene-Fiorese, M., Dâmaso, A. R., and Cheik, N. C. Dieta hiperlipídica e capacidade secretória de insulina em ratos. Rev. Nutr. Vol. 19, Nº 3, 2006, pp. 341-348.
Borba A. J., Rocha, M. G. M, Da Silva, M. F, Tibúrcio, D. T. S, Pereira, A. A. L., Dos Reis, L. C. and Thedei Júnior, G. Dieta hiperlipídico-proteica e obesidade. Rev. Nutr. Vol. 24, Nº 4, 2011, pp. 519-528.
Mahan L. K. and Escott-Stump S. KRAUSE: alimentos, nutrição e dietoterapia. 10ª ed. São Paulo: Roca, p.13, 2002.
Westerpep-Platenga M. S. Effects of energy density of daily food intake on long-term energy intake. Physiology & Behavior. Vol. 81, Nº 5, 2004, pp. 765-771.
Moura, L. P. D., Dalia, R. A., Araújo, M. B. D., Sponton, A. C. D. S., Pauli, J. R., Moura, R. F. D., and Mello, M. A. R. D. Alterações bioquímicas e hepáticas em ratos submetidos à uma dieta hiperlipídica/hiperenergética. Revista de Nutrição, 2012, pp. 685-693.
Yadav, M., Jain, S., Bhardwaj, A., Nagpal, R., Puniya, M., Tomar, R.... and Yadav, H. Biological and medicinal properties of grapes and their bioactive constituents: an update. Journal of Medicinal Food. Vol. 12, Nº 3, 2009, pp. 473-484.
Dani, C., Oliboni, L. S., Pasquali, M. A., Oliveira, M. R., Umezu, F. M., Salvador, M.... and Henriques, J. A. Intake of purple grape juice as a hepatoprotective agent in Wistar rats. Journal of Medicinal Food. Vol. 11, Nº 1, 2008, pp. 127-132.
Nematbakhsh, M., Zolfaghari, B., Eshraghi, F., Safari, T., Pezeshki, Z., and Sorooshzadeh, S. M. A. The effects of unripe grape extract on systemic blood pressure, nitric oxide production, and response to angiotensin II administration. Pharmacognosy research. Vol. 5, Nº 2, 2013, pp. 60.
Quinones, M., Miguel, M., and Aleixandre, A. The polyphenols, naturally occurring compounds with beneficial effects on cardiovascular disease. Nutrición Hospitalaria. Vol. 27, Nº 1, 2011, pp. 76-89.
Deji, N., Kume, S., Araki, S. I., Soumura, M., Sugimoto, T., Isshiki, K.... and Uzu, T. Structural and functional changes in the kidneys of high-fat diet-induced obese mice. American Journal of Physiology-Renal Physiology. Vol. 296, Nº 1, 2009, pp. 118-126.
Petrovski, G., Gurusamy, N., and Das, D. K. Resveratrol in cardiovascular health and disease. Annals of the New York Academy of Sciences. Vol. 1215, Nº 1, 2011, pp. 22-33.
Rodrigues, A. D., Scheffel, T. B., Scola, G., dos Santos, M. T., Fank, B., de Freitas, S. C. V., .. and Salvador, M. Neuroprotective and anticonvulsant effects of organic and conventional purple grape juices on seizures in Wistar rats induced by pentylenetetrazole. Neurochemistry international. Vol. 60, Nº 8, 2012, pp. 799-805, 2012.
Slater, S. J., Seiz, J. L., Cook, A. C., Stagliano, B. A., Buzas, C.J. Inhibition of protein kinase C by resveratrol. Biochim Biophys Acta. Vol. 1637, Nº 1, 2003, pp. 59-69.
Matos, R. S., Baroncini, L. A. V., Précoma, L. B., Winter, G., Lambach, P. H., Kaiber, F., and Précoma, D. B. Resveratrol causes antiatherogenic effects in an animal model of atherosclerosis. Arquivos Brasileiros de Cardiologia. Vol. 98, Nº 2, 2012, pp. 136-142.
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