Proteases are multipurpose group of enzymes which mainly produced from microorganism and used in various industries such as detergent, silver recovery, food, pharmaceutical, leather and textile industries. This work aimed to isolate protease producing bacteria from local environment for use as silver recovery and reuse of polyester from waste X-ray film. Isolation of protease producer was undertaken using skim milk agar medium. A total of 36 protease positive bacteria were isolated from the study area (Arba Minch fish processing site). Five isolates were screened which had relatively higher clear zone and further examined gelatin removal efficiency of their crude enzymes. One isolate designated as Bacillus sp. THZ14 was selected on the basis of gelatin removal efficiency. The crude enzyme from this strain removed gelatin on waste X-ray film at pH 9, 40°C in 40 min. These properties suggest that protease from Bacillus sp. THZ14 could find potential application in silver recovery process and reuse of polyester sheet which have an economic implication.
| Published in | American Journal of BioScience (Volume 5, Issue 5) |
| DOI | 10.11648/j.ajbio.20170505.11 |
| Page(s) | 74-79 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Bacillus sp. THZ14, Enzyme, Gelatin Hydrolysis, Protease, X-ray Films
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APA Style
Temam Abrar Hamza. (2017). Isolation of Protease Producing Bacteria from Soil for Polyester and Silver Recovery from Waste X-ray Film. American Journal of BioScience, 5(5), 74-79. https://doi.org/10.11648/j.ajbio.20170505.11
ACS Style
Temam Abrar Hamza. Isolation of Protease Producing Bacteria from Soil for Polyester and Silver Recovery from Waste X-ray Film. Am. J. BioScience 2017, 5(5), 74-79. doi: 10.11648/j.ajbio.20170505.11
AMA Style
Temam Abrar Hamza. Isolation of Protease Producing Bacteria from Soil for Polyester and Silver Recovery from Waste X-ray Film. Am J BioScience. 2017;5(5):74-79. doi: 10.11648/j.ajbio.20170505.11
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Download@article{10.11648/j.ajbio.20170505.11,
author = {Temam Abrar Hamza},
title = {Isolation of Protease Producing Bacteria from Soil for Polyester and Silver Recovery from Waste X-ray Film},
journal = {American Journal of BioScience},
volume = {5},
number = {5},
pages = {74-79},
doi = {10.11648/j.ajbio.20170505.11},
url = {https://doi.org/10.11648/j.ajbio.20170505.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20170505.11},
abstract = {Proteases are multipurpose group of enzymes which mainly produced from microorganism and used in various industries such as detergent, silver recovery, food, pharmaceutical, leather and textile industries. This work aimed to isolate protease producing bacteria from local environment for use as silver recovery and reuse of polyester from waste X-ray film. Isolation of protease producer was undertaken using skim milk agar medium. A total of 36 protease positive bacteria were isolated from the study area (Arba Minch fish processing site). Five isolates were screened which had relatively higher clear zone and further examined gelatin removal efficiency of their crude enzymes. One isolate designated as Bacillus sp. THZ14 was selected on the basis of gelatin removal efficiency. The crude enzyme from this strain removed gelatin on waste X-ray film at pH 9, 40°C in 40 min. These properties suggest that protease from Bacillus sp. THZ14 could find potential application in silver recovery process and reuse of polyester sheet which have an economic implication.},
year = {2017}
}
TY - JOUR T1 - Isolation of Protease Producing Bacteria from Soil for Polyester and Silver Recovery from Waste X-ray Film AU - Temam Abrar Hamza Y1 - 2017/08/30 PY - 2017 N1 - https://doi.org/10.11648/j.ajbio.20170505.11 DO - 10.11648/j.ajbio.20170505.11 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 74 EP - 79 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20170505.11 AB - Proteases are multipurpose group of enzymes which mainly produced from microorganism and used in various industries such as detergent, silver recovery, food, pharmaceutical, leather and textile industries. This work aimed to isolate protease producing bacteria from local environment for use as silver recovery and reuse of polyester from waste X-ray film. Isolation of protease producer was undertaken using skim milk agar medium. A total of 36 protease positive bacteria were isolated from the study area (Arba Minch fish processing site). Five isolates were screened which had relatively higher clear zone and further examined gelatin removal efficiency of their crude enzymes. One isolate designated as Bacillus sp. THZ14 was selected on the basis of gelatin removal efficiency. The crude enzyme from this strain removed gelatin on waste X-ray film at pH 9, 40°C in 40 min. These properties suggest that protease from Bacillus sp. THZ14 could find potential application in silver recovery process and reuse of polyester sheet which have an economic implication. VL - 5 IS - 5 ER -
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