Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling

Wenjing Huang; Guangzhu Yang; Fan Li; Liping Zheng; Jun Ma

doi:doi:10.11648/j.ajpb.20180302.11

| Peer-Reviewed

Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling

Wenjing Huang, Guangzhu Yang, Fan Li, Liping Zheng, Jun Ma

Published in American Journal of Plant Biology (Volume 3, Issue 2)

Received: 22 August 2018 Accepted: 12 September 2018 Published: 10 October 2018

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. The results showed that in the culture medium (bud induction medium LS + 4.0mg/L 6-BA + 0.2 mg/L NAA, proliferation medium LS + 2.5mg/L 6-BA + 0.1mg/L NAA , rooting medium 1/2 LS + 0.5mg/L NAA + 1.0mg/L IAA), with 30g/L sugar and 9g/L agar, pH 5.8, autoclave 121°C, 20 minuets. At the same culture conditions, 21±1°C temperature, 12 hours of light, 12 hours of dark and same illumination time. The LED treatments were better than the fluorescent light of the germination; LED r/b ratio 5:5, was the best on germination effect, the height of seedling was 1.84cm and 10 leaves on 50 days of cultivation; LED r/b ratio 6:4, was the best on the proliferation stage and rooting effect stage, the height of seedling was 4.15cm on 45 days of proliferation and the main-root length was 5.97cm, and the height of seedling was 6.38cm on 45 days of rooting stage; followed by LED r/b 7:3 and 5:5. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, also saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively. So it was reduced of the tissue culture production in a certain extent.

Published in	American Journal of Plant Biology (Volume 3, Issue 2)
DOI	10.11648/j.ajpb.20180302.11
Page(s)	17-20
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

LED Apple Root-Stock Tissue Culture Seedling Growth and Development

References

[1]	Briand CH, Hicks GS (1989). Micropropagation of the cold-hardy apple rootstock KSC-3: a morphological analysis. Can J Plant Sci, 69: 555-564
[2]	Kermani MJ, Hosseini ZS, Habashi AA (2009). A refined tissue culture medium for in vitro proliferation of apple roots-tocks. Acta Hort (ISHS), 829: 313-318
[3]	Sicurani M, Piccioni E, Standardi A (2001). Micropropagation and preparation of synthetic seed in M. 26 apple rootstock I: attempts towards saving labor in the production of adventitious shoot tips suitable for encapsulation. Plant Cell Tiss Organ Cult, 66: 207-216
[4]	Yaseen M, Ahmad T, Abbasi NA, Hafiz IA (2009). Assessment of apple rootstocks M9 and M26 for in vitro rooting potential using different carbon sources. Pak J Bot, 41 (2): 769-781
[5]	Wenke Liu, Qichang Yang, lingling Wei, LED light source and its horticultural application[M], Agricultural Science and Technology Press of China , 2012
[6]	Tissue culture of Apple Dwarf rootstock 'JM7' and its adventitious shoot regeneration in vitro, Plant Physiology Journal 2014, 50 (6): 779-78
[7]	Liu X. Y., Chang T. T., Guo S. R., et al. Effect of different light quality of LED on growth and photosynthetic character in cherry tomato seedling. Acta Horticulturae, 2011, 907: 325-330
[8]	Yanagi T., Okamoto K., Takita S. Effect of blue and red light intensity on photosynthetic rate of strawberry leaves. Acta Horticultruae, 1996, 440: 371-376
[9]	Jao R. C., Lai C. C., Fang W., et al. Effects of red light on the growth of Zantedeschia plant lets in vitro and tuber formation using light-emitting diodes. HorScience, 2005, 40(2): 436-438
[10]	Wenjing Huang, Guangzhu Yang, Liping Zheng, Jun Ma, Effects of different light quality on growth and development of Pitaya tissue culture seedlings, China Fruits, 2014, (4)
[11]	Rueychi J., Wei F. Growth of potato plantlets in vitro is different when provided concurrent versus alternating blue and red light photoperiods. HortScience, 2004, 39(2): 380-382

Cite This Article

Plain Text BibTeX RIS

APA Style

Wenjing Huang, Guangzhu Yang, Fan Li, Liping Zheng, Jun Ma. (2018). Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling. American Journal of Plant Biology, 3(2), 17-20. https://doi.org/10.11648/j.ajpb.20180302.11

Copy | Download

ACS Style

Wenjing Huang; Guangzhu Yang; Fan Li; Liping Zheng; Jun Ma. Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling. Am. J. Plant Biol. 2018, 3(2), 17-20. doi: 10.11648/j.ajpb.20180302.11

Copy | Download

AMA Style

Wenjing Huang, Guangzhu Yang, Fan Li, Liping Zheng, Jun Ma. Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling. Am J Plant Biol. 2018;3(2):17-20. doi: 10.11648/j.ajpb.20180302.11

Copy | Download

@article{10.11648/j.ajpb.20180302.11,
  author = {Wenjing Huang and Guangzhu Yang and Fan Li and Liping Zheng and Jun Ma},
  title = {Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling},
  journal = {American Journal of Plant Biology},
  volume = {3},
  number = {2},
  pages = {17-20},
  doi = {10.11648/j.ajpb.20180302.11},
  url = {https://doi.org/10.11648/j.ajpb.20180302.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20180302.11},
  abstract = {Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. The results showed that in the culture medium (bud induction medium LS + 4.0mg/L 6-BA + 0.2 mg/L NAA, proliferation medium LS + 2.5mg/L 6-BA + 0.1mg/L NAA , rooting medium 1/2 LS + 0.5mg/L NAA + 1.0mg/L IAA), with 30g/L sugar and 9g/L agar, pH 5.8, autoclave 121°C, 20 minuets. At the same culture conditions, 21±1°C temperature, 12 hours of light, 12 hours of dark and same illumination time. The LED treatments were better than the fluorescent light of the germination; LED r/b ratio 5:5, was the best on germination effect, the height of seedling was 1.84cm and 10 leaves on 50 days of cultivation; LED r/b ratio 6:4, was the best on the proliferation stage and rooting effect stage, the height of seedling was 4.15cm on 45 days of proliferation and the main-root length was 5.97cm, and the height of seedling was 6.38cm on 45 days of rooting stage; followed by LED r/b 7:3 and 5:5. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, also saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively. So it was reduced of the tissue culture production in a certain extent.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling
AU  - Wenjing Huang
AU  - Guangzhu Yang
AU  - Fan Li
AU  - Liping Zheng
AU  - Jun Ma
Y1  - 2018/10/10
PY  - 2018
N1  - https://doi.org/10.11648/j.ajpb.20180302.11
DO  - 10.11648/j.ajpb.20180302.11
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 17
EP  - 20
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.20180302.11
AB  - Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. The results showed that in the culture medium (bud induction medium LS + 4.0mg/L 6-BA + 0.2 mg/L NAA, proliferation medium LS + 2.5mg/L 6-BA + 0.1mg/L NAA , rooting medium 1/2 LS + 0.5mg/L NAA + 1.0mg/L IAA), with 30g/L sugar and 9g/L agar, pH 5.8, autoclave 121°C, 20 minuets. At the same culture conditions, 21±1°C temperature, 12 hours of light, 12 hours of dark and same illumination time. The LED treatments were better than the fluorescent light of the germination; LED r/b ratio 5:5, was the best on germination effect, the height of seedling was 1.84cm and 10 leaves on 50 days of cultivation; LED r/b ratio 6:4, was the best on the proliferation stage and rooting effect stage, the height of seedling was 4.15cm on 45 days of proliferation and the main-root length was 5.97cm, and the height of seedling was 6.38cm on 45 days of rooting stage; followed by LED r/b 7:3 and 5:5. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, also saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively. So it was reduced of the tissue culture production in a certain extent.
VL  - 3
IS  - 2
ER  -

Copy | Download

Author Information

Wenjing Huang

Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Guangzhu Yang

Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Fan Li

Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Liping Zheng

Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Jun Ma

Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Wenjing Huang, Guangzhu Yang, Fan Li, Liping Zheng, Jun Ma. (2018). Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling. American Journal of Plant Biology, 3(2), 17-20. https://doi.org/10.11648/j.ajpb.20180302.11

Copy | Download

ACS Style

Wenjing Huang; Guangzhu Yang; Fan Li; Liping Zheng; Jun Ma. Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling. Am. J. Plant Biol. 2018, 3(2), 17-20. doi: 10.11648/j.ajpb.20180302.11

Copy | Download

AMA Style

Wenjing Huang, Guangzhu Yang, Fan Li, Liping Zheng, Jun Ma. Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling. Am J Plant Biol. 2018;3(2):17-20. doi: 10.11648/j.ajpb.20180302.11

Copy | Download

@article{10.11648/j.ajpb.20180302.11,
  author = {Wenjing Huang and Guangzhu Yang and Fan Li and Liping Zheng and Jun Ma},
  title = {Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling},
  journal = {American Journal of Plant Biology},
  volume = {3},
  number = {2},
  pages = {17-20},
  doi = {10.11648/j.ajpb.20180302.11},
  url = {https://doi.org/10.11648/j.ajpb.20180302.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20180302.11},
  abstract = {Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. The results showed that in the culture medium (bud induction medium LS + 4.0mg/L 6-BA + 0.2 mg/L NAA, proliferation medium LS + 2.5mg/L 6-BA + 0.1mg/L NAA , rooting medium 1/2 LS + 0.5mg/L NAA + 1.0mg/L IAA), with 30g/L sugar and 9g/L agar, pH 5.8, autoclave 121°C, 20 minuets. At the same culture conditions, 21±1°C temperature, 12 hours of light, 12 hours of dark and same illumination time. The LED treatments were better than the fluorescent light of the germination; LED r/b ratio 5:5, was the best on germination effect, the height of seedling was 1.84cm and 10 leaves on 50 days of cultivation; LED r/b ratio 6:4, was the best on the proliferation stage and rooting effect stage, the height of seedling was 4.15cm on 45 days of proliferation and the main-root length was 5.97cm, and the height of seedling was 6.38cm on 45 days of rooting stage; followed by LED r/b 7:3 and 5:5. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, also saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively. So it was reduced of the tissue culture production in a certain extent.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling
AU  - Wenjing Huang
AU  - Guangzhu Yang
AU  - Fan Li
AU  - Liping Zheng
AU  - Jun Ma
Y1  - 2018/10/10
PY  - 2018
N1  - https://doi.org/10.11648/j.ajpb.20180302.11
DO  - 10.11648/j.ajpb.20180302.11
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 17
EP  - 20
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.20180302.11
AB  - Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. The results showed that in the culture medium (bud induction medium LS + 4.0mg/L 6-BA + 0.2 mg/L NAA, proliferation medium LS + 2.5mg/L 6-BA + 0.1mg/L NAA , rooting medium 1/2 LS + 0.5mg/L NAA + 1.0mg/L IAA), with 30g/L sugar and 9g/L agar, pH 5.8, autoclave 121°C, 20 minuets. At the same culture conditions, 21±1°C temperature, 12 hours of light, 12 hours of dark and same illumination time. The LED treatments were better than the fluorescent light of the germination; LED r/b ratio 5:5, was the best on germination effect, the height of seedling was 1.84cm and 10 leaves on 50 days of cultivation; LED r/b ratio 6:4, was the best on the proliferation stage and rooting effect stage, the height of seedling was 4.15cm on 45 days of proliferation and the main-root length was 5.97cm, and the height of seedling was 6.38cm on 45 days of rooting stage; followed by LED r/b 7:3 and 5:5. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, also saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively. So it was reduced of the tissue culture production in a certain extent.
VL  - 3
IS  - 2
ER  -

Copy | Download