Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from <i>Justicia brandegeana</i>

Ayman Aly Shehawy; Magda Tohamy Ibrahim; Abdulmajeed Fahad Alrefaei; Hamed Abdel-Dayem Mohamed; Enas Sameer Aboutaleb; Sultan Kadasah

doi:doi:10.11648/j.ijnfs.20221105.17

Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana

Ayman Aly Shehawy, Magda Tohamy Ibrahim, Abdulmajeed Fahad Alrefaei, Hamed Abdel-Dayem Mohamed, Enas Sameer Aboutaleb, Sultan Kadasah

Published in International Journal of Nutrition and Food Sciences (Volume 11, Issue 5)

Received: 3 October 2022 Accepted: 21 October 2022 Published: 27 October 2022

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Abstract

Several species of Justicia (Acanthaceae) are widely used in folk medicine. Also, the plants are used in treatment of central nervous system disorders and epilepsy. On the other hand, some species of this genus are used popularly in headache and fever treatment, in addition to their role as sedative, analgesic, and anti-tumor effect. This study was carried out for isolation and purification of different metabolites as well as assessment the cytotoxicity effect of the major one; β-sitosterol-β-D-glucoside against different type of cancer cell lines. An aqueous methanol extract of J. brandegeana has led to the isolation and identification of six known metabolites: free disaccharides known as 4-O-(α-D-glucopyranosyl)- β-D-glucopyranose (1), methyl-4-hydroxybenzoate (2), p-hydroxybenzoic acid (3), Quercetin 3-O-β-D-4C1-galactopyranoside (4), β-Sitosterol-β-D-glucoside (5) and Lupeol (6) for the first time. Their structure elucidation was based on different spectroscopic analyses. Indeed, Cytotoxic activity of the major constituent namely; β-Sitosterol-β-D-glucoside was evaluated against seven types of cancer cell lines namely; Breast carcinoma (MCF-7), Hepatocellular carcinoma (HepG-2), Colon carcinoma (HCT-116), Lung carcinoma (A-549), Prostate carcinoma (PC-3), Larynx carcinoma HEP-2 and Cervical carcinoma (HELA) cell lines, the results revealed that the cytotoxicity ranged from 0.57-57.25, 3.72-65.08, 5.94-68.51, 8.22-76.15, 0.0-60.13, 0.0-38.62, and 0.0-25.81% in case of breast carcinoma, hepatocellular carcinoma, colon carcinoma, lung carcinoma, prostate carcinoma, larynx carcinoma, and cervical carcinoma cell lines respectively. Thus, β-sitosterol-β-D-glucoside could be recommended as anti-cancer (Lung carcinoma) after determining the safe dose for human use.

Published in	International Journal of Nutrition and Food Sciences (Volume 11, Issue 5)
DOI	10.11648/j.ijnfs.20221105.17
Page(s)	159-169
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lupeol, β-Sitosterol-β-D-glucoside, Justicia brandegeana, Isolation, Bioactive Natural Products Activity

References

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Ayman Aly Shehawy, Magda Tohamy Ibrahim, Abdulmajeed Fahad Alrefaei, Hamed Abdel-Dayem Mohamed, Enas Sameer Aboutaleb, et al. (2022). Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana. International Journal of Nutrition and Food Sciences, 11(5), 159-169. https://doi.org/10.11648/j.ijnfs.20221105.17

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ACS Style

Ayman Aly Shehawy; Magda Tohamy Ibrahim; Abdulmajeed Fahad Alrefaei; Hamed Abdel-Dayem Mohamed; Enas Sameer Aboutaleb, et al. Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana. Int. J. Nutr. Food Sci. 2022, 11(5), 159-169. doi: 10.11648/j.ijnfs.20221105.17

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AMA Style

Ayman Aly Shehawy, Magda Tohamy Ibrahim, Abdulmajeed Fahad Alrefaei, Hamed Abdel-Dayem Mohamed, Enas Sameer Aboutaleb, et al. Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana. Int J Nutr Food Sci. 2022;11(5):159-169. doi: 10.11648/j.ijnfs.20221105.17

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@article{10.11648/j.ijnfs.20221105.17,
  author = {Ayman Aly Shehawy and Magda Tohamy Ibrahim and Abdulmajeed Fahad Alrefaei and Hamed Abdel-Dayem Mohamed and Enas Sameer Aboutaleb and Sultan Kadasah},
  title = {Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {11},
  number = {5},
  pages = {159-169},
  doi = {10.11648/j.ijnfs.20221105.17},
  url = {https://doi.org/10.11648/j.ijnfs.20221105.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20221105.17},
  abstract = {Several species of Justicia (Acanthaceae) are widely used in folk medicine. Also, the plants are used in treatment of central nervous system disorders and epilepsy. On the other hand, some species of this genus are used popularly in headache and fever treatment, in addition to their role as sedative, analgesic, and anti-tumor effect. This study was carried out for isolation and purification of different metabolites as well as assessment the cytotoxicity effect of the major one; β-sitosterol-β-D-glucoside against different type of cancer cell lines. An aqueous methanol extract of J. brandegeana has led to the isolation and identification of six known metabolites: free disaccharides known as 4-O-(α-D-glucopyranosyl)- β-D-glucopyranose (1), methyl-4-hydroxybenzoate (2), p-hydroxybenzoic acid (3), Quercetin 3-O-β-D-4C1-galactopyranoside (4), β-Sitosterol-β-D-glucoside (5) and Lupeol (6) for the first time. Their structure elucidation was based on different spectroscopic analyses. Indeed, Cytotoxic activity of the major constituent namely; β-Sitosterol-β-D-glucoside was evaluated against seven types of cancer cell lines namely; Breast carcinoma (MCF-7), Hepatocellular carcinoma (HepG-2), Colon carcinoma (HCT-116), Lung carcinoma (A-549), Prostate carcinoma (PC-3), Larynx carcinoma HEP-2 and Cervical carcinoma (HELA) cell lines, the results revealed that the cytotoxicity ranged from 0.57-57.25, 3.72-65.08, 5.94-68.51, 8.22-76.15, 0.0-60.13, 0.0-38.62, and 0.0-25.81% in case of breast carcinoma, hepatocellular carcinoma, colon carcinoma, lung carcinoma, prostate carcinoma, larynx carcinoma, and cervical carcinoma cell lines respectively. Thus, β-sitosterol-β-D-glucoside could be recommended as anti-cancer (Lung carcinoma) after determining the safe dose for human use.},
 year = {2022}
}

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TY  - JOUR
T1  - Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana
AU  - Ayman Aly Shehawy
AU  - Magda Tohamy Ibrahim
AU  - Abdulmajeed Fahad Alrefaei
AU  - Hamed Abdel-Dayem Mohamed
AU  - Enas Sameer Aboutaleb
AU  - Sultan Kadasah
Y1  - 2022/10/27
PY  - 2022
N1  - https://doi.org/10.11648/j.ijnfs.20221105.17
DO  - 10.11648/j.ijnfs.20221105.17
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 159
EP  - 169
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20221105.17
AB  - Several species of Justicia (Acanthaceae) are widely used in folk medicine. Also, the plants are used in treatment of central nervous system disorders and epilepsy. On the other hand, some species of this genus are used popularly in headache and fever treatment, in addition to their role as sedative, analgesic, and anti-tumor effect. This study was carried out for isolation and purification of different metabolites as well as assessment the cytotoxicity effect of the major one; β-sitosterol-β-D-glucoside against different type of cancer cell lines. An aqueous methanol extract of J. brandegeana has led to the isolation and identification of six known metabolites: free disaccharides known as 4-O-(α-D-glucopyranosyl)- β-D-glucopyranose (1), methyl-4-hydroxybenzoate (2), p-hydroxybenzoic acid (3), Quercetin 3-O-β-D-4C1-galactopyranoside (4), β-Sitosterol-β-D-glucoside (5) and Lupeol (6) for the first time. Their structure elucidation was based on different spectroscopic analyses. Indeed, Cytotoxic activity of the major constituent namely; β-Sitosterol-β-D-glucoside was evaluated against seven types of cancer cell lines namely; Breast carcinoma (MCF-7), Hepatocellular carcinoma (HepG-2), Colon carcinoma (HCT-116), Lung carcinoma (A-549), Prostate carcinoma (PC-3), Larynx carcinoma HEP-2 and Cervical carcinoma (HELA) cell lines, the results revealed that the cytotoxicity ranged from 0.57-57.25, 3.72-65.08, 5.94-68.51, 8.22-76.15, 0.0-60.13, 0.0-38.62, and 0.0-25.81% in case of breast carcinoma, hepatocellular carcinoma, colon carcinoma, lung carcinoma, prostate carcinoma, larynx carcinoma, and cervical carcinoma cell lines respectively. Thus, β-sitosterol-β-D-glucoside could be recommended as anti-cancer (Lung carcinoma) after determining the safe dose for human use.
VL  - 11
IS  - 5
ER  -

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Author Information

Ayman Aly Shehawy

Green Pest Control Lab., Plant Protection Research Institute, Agricultural Research Centre, Giza, Egypt
Magda Tohamy Ibrahim

Pharmacognosy Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
Abdulmajeed Fahad Alrefaei

Department of Biology, Jamoum University College, U mm Al-Qura University, Makkah, Saudi Arabia
Hamed Abdel-Dayem Mohamed

Green Pest Control Lab., Plant Protection Research Institute, Agricultural Research Centre, Giza, Egypt
Enas Sameer Aboutaleb

Pharmacognosy Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
Sultan Kadasah

Department of Biology, Faculty of Science, University of Bisha, Bisha, Saudi Arabia

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APA Style

Ayman Aly Shehawy, Magda Tohamy Ibrahim, Abdulmajeed Fahad Alrefaei, Hamed Abdel-Dayem Mohamed, Enas Sameer Aboutaleb, et al. (2022). Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana. International Journal of Nutrition and Food Sciences, 11(5), 159-169. https://doi.org/10.11648/j.ijnfs.20221105.17

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ACS Style

Ayman Aly Shehawy; Magda Tohamy Ibrahim; Abdulmajeed Fahad Alrefaei; Hamed Abdel-Dayem Mohamed; Enas Sameer Aboutaleb, et al. Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana. Int. J. Nutr. Food Sci. 2022, 11(5), 159-169. doi: 10.11648/j.ijnfs.20221105.17

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AMA Style

Ayman Aly Shehawy, Magda Tohamy Ibrahim, Abdulmajeed Fahad Alrefaei, Hamed Abdel-Dayem Mohamed, Enas Sameer Aboutaleb, et al. Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana. Int J Nutr Food Sci. 2022;11(5):159-169. doi: 10.11648/j.ijnfs.20221105.17

Copy | Download

@article{10.11648/j.ijnfs.20221105.17,
  author = {Ayman Aly Shehawy and Magda Tohamy Ibrahim and Abdulmajeed Fahad Alrefaei and Hamed Abdel-Dayem Mohamed and Enas Sameer Aboutaleb and Sultan Kadasah},
  title = {Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {11},
  number = {5},
  pages = {159-169},
  doi = {10.11648/j.ijnfs.20221105.17},
  url = {https://doi.org/10.11648/j.ijnfs.20221105.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20221105.17},
  abstract = {Several species of Justicia (Acanthaceae) are widely used in folk medicine. Also, the plants are used in treatment of central nervous system disorders and epilepsy. On the other hand, some species of this genus are used popularly in headache and fever treatment, in addition to their role as sedative, analgesic, and anti-tumor effect. This study was carried out for isolation and purification of different metabolites as well as assessment the cytotoxicity effect of the major one; β-sitosterol-β-D-glucoside against different type of cancer cell lines. An aqueous methanol extract of J. brandegeana has led to the isolation and identification of six known metabolites: free disaccharides known as 4-O-(α-D-glucopyranosyl)- β-D-glucopyranose (1), methyl-4-hydroxybenzoate (2), p-hydroxybenzoic acid (3), Quercetin 3-O-β-D-4C1-galactopyranoside (4), β-Sitosterol-β-D-glucoside (5) and Lupeol (6) for the first time. Their structure elucidation was based on different spectroscopic analyses. Indeed, Cytotoxic activity of the major constituent namely; β-Sitosterol-β-D-glucoside was evaluated against seven types of cancer cell lines namely; Breast carcinoma (MCF-7), Hepatocellular carcinoma (HepG-2), Colon carcinoma (HCT-116), Lung carcinoma (A-549), Prostate carcinoma (PC-3), Larynx carcinoma HEP-2 and Cervical carcinoma (HELA) cell lines, the results revealed that the cytotoxicity ranged from 0.57-57.25, 3.72-65.08, 5.94-68.51, 8.22-76.15, 0.0-60.13, 0.0-38.62, and 0.0-25.81% in case of breast carcinoma, hepatocellular carcinoma, colon carcinoma, lung carcinoma, prostate carcinoma, larynx carcinoma, and cervical carcinoma cell lines respectively. Thus, β-sitosterol-β-D-glucoside could be recommended as anti-cancer (Lung carcinoma) after determining the safe dose for human use.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Assessment of Antitumor Activity of β-sitosterol-β-D-glucoside Isolated and Identified from Justicia brandegeana
AU  - Ayman Aly Shehawy
AU  - Magda Tohamy Ibrahim
AU  - Abdulmajeed Fahad Alrefaei
AU  - Hamed Abdel-Dayem Mohamed
AU  - Enas Sameer Aboutaleb
AU  - Sultan Kadasah
Y1  - 2022/10/27
PY  - 2022
N1  - https://doi.org/10.11648/j.ijnfs.20221105.17
DO  - 10.11648/j.ijnfs.20221105.17
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 159
EP  - 169
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20221105.17
AB  - Several species of Justicia (Acanthaceae) are widely used in folk medicine. Also, the plants are used in treatment of central nervous system disorders and epilepsy. On the other hand, some species of this genus are used popularly in headache and fever treatment, in addition to their role as sedative, analgesic, and anti-tumor effect. This study was carried out for isolation and purification of different metabolites as well as assessment the cytotoxicity effect of the major one; β-sitosterol-β-D-glucoside against different type of cancer cell lines. An aqueous methanol extract of J. brandegeana has led to the isolation and identification of six known metabolites: free disaccharides known as 4-O-(α-D-glucopyranosyl)- β-D-glucopyranose (1), methyl-4-hydroxybenzoate (2), p-hydroxybenzoic acid (3), Quercetin 3-O-β-D-4C1-galactopyranoside (4), β-Sitosterol-β-D-glucoside (5) and Lupeol (6) for the first time. Their structure elucidation was based on different spectroscopic analyses. Indeed, Cytotoxic activity of the major constituent namely; β-Sitosterol-β-D-glucoside was evaluated against seven types of cancer cell lines namely; Breast carcinoma (MCF-7), Hepatocellular carcinoma (HepG-2), Colon carcinoma (HCT-116), Lung carcinoma (A-549), Prostate carcinoma (PC-3), Larynx carcinoma HEP-2 and Cervical carcinoma (HELA) cell lines, the results revealed that the cytotoxicity ranged from 0.57-57.25, 3.72-65.08, 5.94-68.51, 8.22-76.15, 0.0-60.13, 0.0-38.62, and 0.0-25.81% in case of breast carcinoma, hepatocellular carcinoma, colon carcinoma, lung carcinoma, prostate carcinoma, larynx carcinoma, and cervical carcinoma cell lines respectively. Thus, β-sitosterol-β-D-glucoside could be recommended as anti-cancer (Lung carcinoma) after determining the safe dose for human use.
VL  - 11
IS  - 5
ER  -

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