Anti- oxidants are crucial to human health. They have capacity to neutralize the free radicals responsible for giving birth to a variety of diseases. A diet rich in foods with high levels of anti-oxidants is associated with longevity and good health. We tried to replace the chemical anti-oxidants by herbal anti-oxidants. In plants, the anti-oxidants are present as flavonoids,so the flavonoids in Urena lobata were isolated , identified, quantitatively estimated and tested for their anti-oxidant activity.The flavonoids were extracted from the stem and the leaves separately to know the relative contents. Quercetin and Kaempferol were found to exist in the plant material.These were qualitatively tested by the method of Thin Layer Chromatography (TLC). The isolation, purification and quantitative content determination was performed by preparative thin layer chromatography (PTLC). The anti-oxidant activity of the methanolic plant extract was examined by DPPH radical scavenging activity method. The anti-oxidant ability of the plant extract was found comparable with Vitamin C.
Published in | Journal of Plant Sciences (Volume 2, Issue 6) |
DOI | 10.11648/j.jps.20140206.16 |
Page(s) | 299-303 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Urena Lobata, Quercetin, Kaempferol, Myrecetin, Silica Gel-G, Ascorbic Acid and DPPH
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APA Style
Dixa Singh, V. S. Singh. (2014). Urena lobata: A Green Source of Anti-Oxidant. Journal of Plant Sciences, 2(6), 299-303. https://doi.org/10.11648/j.jps.20140206.16
ACS Style
Dixa Singh; V. S. Singh. Urena lobata: A Green Source of Anti-Oxidant. J. Plant Sci. 2014, 2(6), 299-303. doi: 10.11648/j.jps.20140206.16
AMA Style
Dixa Singh, V. S. Singh. Urena lobata: A Green Source of Anti-Oxidant. J Plant Sci. 2014;2(6):299-303. doi: 10.11648/j.jps.20140206.16
@article{10.11648/j.jps.20140206.16, author = {Dixa Singh and V. S. Singh}, title = {Urena lobata: A Green Source of Anti-Oxidant}, journal = {Journal of Plant Sciences}, volume = {2}, number = {6}, pages = {299-303}, doi = {10.11648/j.jps.20140206.16}, url = {https://doi.org/10.11648/j.jps.20140206.16}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20140206.16}, abstract = {Anti- oxidants are crucial to human health. They have capacity to neutralize the free radicals responsible for giving birth to a variety of diseases. A diet rich in foods with high levels of anti-oxidants is associated with longevity and good health. We tried to replace the chemical anti-oxidants by herbal anti-oxidants. In plants, the anti-oxidants are present as flavonoids,so the flavonoids in Urena lobata were isolated , identified, quantitatively estimated and tested for their anti-oxidant activity.The flavonoids were extracted from the stem and the leaves separately to know the relative contents. Quercetin and Kaempferol were found to exist in the plant material.These were qualitatively tested by the method of Thin Layer Chromatography (TLC). The isolation, purification and quantitative content determination was performed by preparative thin layer chromatography (PTLC). The anti-oxidant activity of the methanolic plant extract was examined by DPPH radical scavenging activity method. The anti-oxidant ability of the plant extract was found comparable with Vitamin C.}, year = {2014} }
TY - JOUR T1 - Urena lobata: A Green Source of Anti-Oxidant AU - Dixa Singh AU - V. S. Singh Y1 - 2014/12/17 PY - 2014 N1 - https://doi.org/10.11648/j.jps.20140206.16 DO - 10.11648/j.jps.20140206.16 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 299 EP - 303 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20140206.16 AB - Anti- oxidants are crucial to human health. They have capacity to neutralize the free radicals responsible for giving birth to a variety of diseases. A diet rich in foods with high levels of anti-oxidants is associated with longevity and good health. We tried to replace the chemical anti-oxidants by herbal anti-oxidants. In plants, the anti-oxidants are present as flavonoids,so the flavonoids in Urena lobata were isolated , identified, quantitatively estimated and tested for their anti-oxidant activity.The flavonoids were extracted from the stem and the leaves separately to know the relative contents. Quercetin and Kaempferol were found to exist in the plant material.These were qualitatively tested by the method of Thin Layer Chromatography (TLC). The isolation, purification and quantitative content determination was performed by preparative thin layer chromatography (PTLC). The anti-oxidant activity of the methanolic plant extract was examined by DPPH radical scavenging activity method. The anti-oxidant ability of the plant extract was found comparable with Vitamin C. VL - 2 IS - 6 ER -