Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria

Gidado Rose Suniso Maxwell; Etim Veronica Archibong; Nweke Ogechi; Iloh Andrew Chibuzor; Isu Rosemary Nennaya; Solomon Bamidele Ogbe

doi:doi:10.11648/j.ajbio.20160405.11

Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria

Gidado Rose Suniso Maxwell, Etim Veronica Archibong, Nweke Ogechi, Iloh Andrew Chibuzor, Isu Rosemary Nennaya, Solomon Bamidele Ogbe

Published in American Journal of BioScience (Volume 4, Issue 5)

Received: 25 April 2016 Accepted: 25 August 2016 Published: 12 September 2016

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Abstract

Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.

Published in	American Journal of BioScience (Volume 4, Issue 5)
DOI	10.11648/j.ajbio.20160405.11
Page(s)	58-63
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Ethanol, Ethanol Tolerance, Saccharomyces, Non-saccharomyces, Pichia kudriavzevii

References

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APA Style

Gidado Rose Suniso Maxwell, Etim Veronica Archibong, Nweke Ogechi, Iloh Andrew Chibuzor, Isu Rosemary Nennaya, et al. (2016). Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. American Journal of BioScience, 4(5), 58-63. https://doi.org/10.11648/j.ajbio.20160405.11

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ACS Style

Gidado Rose Suniso Maxwell; Etim Veronica Archibong; Nweke Ogechi; Iloh Andrew Chibuzor; Isu Rosemary Nennaya, et al. Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. Am. J. BioScience 2016, 4(5), 58-63. doi: 10.11648/j.ajbio.20160405.11

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AMA Style

Gidado Rose Suniso Maxwell, Etim Veronica Archibong, Nweke Ogechi, Iloh Andrew Chibuzor, Isu Rosemary Nennaya, et al. Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. Am J BioScience. 2016;4(5):58-63. doi: 10.11648/j.ajbio.20160405.11

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@article{10.11648/j.ajbio.20160405.11,
  author = {Gidado Rose Suniso Maxwell and Etim Veronica Archibong and Nweke Ogechi and Iloh Andrew Chibuzor and Isu Rosemary Nennaya and Solomon Bamidele Ogbe},
  title = {Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria},
  journal = {American Journal of BioScience},
  volume = {4},
  number = {5},
  pages = {58-63},
  doi = {10.11648/j.ajbio.20160405.11},
  url = {https://doi.org/10.11648/j.ajbio.20160405.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20160405.11},
  abstract = {Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.},
 year = {2016}
}

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TY  - JOUR
T1  - Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria
AU  - Gidado Rose Suniso Maxwell
AU  - Etim Veronica Archibong
AU  - Nweke Ogechi
AU  - Iloh Andrew Chibuzor
AU  - Isu Rosemary Nennaya
AU  - Solomon Bamidele Ogbe
Y1  - 2016/09/12
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbio.20160405.11
DO  - 10.11648/j.ajbio.20160405.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 58
EP  - 63
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20160405.11
AB  - Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.
VL  - 4
IS  - 5
ER  -

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Author Information

Gidado Rose Suniso Maxwell

Agricultural Biotechnology Department, National Biotechnology Development Agency (NABDA), Abuja, Nigeria
Etim Veronica Archibong

Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria
Nweke Ogechi

Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria
Iloh Andrew Chibuzor

Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria
Isu Rosemary Nennaya

Department of Biological Sciences, University of Abuja, Abuja, Nigeria
Solomon Bamidele Ogbe

Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Nigeria

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Plain Text BibTeX RIS

APA Style

Gidado Rose Suniso Maxwell, Etim Veronica Archibong, Nweke Ogechi, Iloh Andrew Chibuzor, Isu Rosemary Nennaya, et al. (2016). Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. American Journal of BioScience, 4(5), 58-63. https://doi.org/10.11648/j.ajbio.20160405.11

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ACS Style

Gidado Rose Suniso Maxwell; Etim Veronica Archibong; Nweke Ogechi; Iloh Andrew Chibuzor; Isu Rosemary Nennaya, et al. Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. Am. J. BioScience 2016, 4(5), 58-63. doi: 10.11648/j.ajbio.20160405.11

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AMA Style

Gidado Rose Suniso Maxwell, Etim Veronica Archibong, Nweke Ogechi, Iloh Andrew Chibuzor, Isu Rosemary Nennaya, et al. Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. Am J BioScience. 2016;4(5):58-63. doi: 10.11648/j.ajbio.20160405.11

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@article{10.11648/j.ajbio.20160405.11,
  author = {Gidado Rose Suniso Maxwell and Etim Veronica Archibong and Nweke Ogechi and Iloh Andrew Chibuzor and Isu Rosemary Nennaya and Solomon Bamidele Ogbe},
  title = {Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria},
  journal = {American Journal of BioScience},
  volume = {4},
  number = {5},
  pages = {58-63},
  doi = {10.11648/j.ajbio.20160405.11},
  url = {https://doi.org/10.11648/j.ajbio.20160405.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20160405.11},
  abstract = {Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.},
 year = {2016}
}

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TY  - JOUR
T1  - Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria
AU  - Gidado Rose Suniso Maxwell
AU  - Etim Veronica Archibong
AU  - Nweke Ogechi
AU  - Iloh Andrew Chibuzor
AU  - Isu Rosemary Nennaya
AU  - Solomon Bamidele Ogbe
Y1  - 2016/09/12
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbio.20160405.11
DO  - 10.11648/j.ajbio.20160405.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 58
EP  - 63
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20160405.11
AB  - Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.
VL  - 4
IS  - 5
ER  -

Copy | Download